Abstract: Pulse polarographic determination of anthraquinone in Polygonum cillinerve (Nakai) Ohwi was reported.The sample was extracted with methanol and separated with thin layer chromatography. The zones of emodin and physcion were removed from the plate, and analysed by pulse polarographic method. 0.1 M lithium chloride solution was used as supporting electrolyte. The peak potential was -0.75 V (vs SEC)The current was proportional to the concentration in the range of 6.4×10^-6～3.1×10^-5 M. The method is accurate and sensitive. The error is<2%.