Abstract: This paper reports the methods of measurement of percentage of drug entrapment and drug content in polyphase liposome of the intravenous injection (139). The percentage of oleic acid entrapped in polyphase liposome of the intravenous injection (139) is determined by the method of gel filtration using column of Sephadex G-50, Qw=94.2% (mean of 7 batches).Moreover, the percentage of drug entrapment by volume of liposome in the intravenous injection (139) is also determined by the method of microscopic photography and Coulter eounter(TAII), Qv= 97.1%(mean of 10 batches).The main influence factors for percentage of drug entrapped in liposome are discussed.