Abstract: Three kinds of biflavone(ginkgetin, isoginkgetin and bilobetin ) were isolatedfrom Ginkgko biloba L.and were determined by reversed phase HPLC using anthracene as internalstandard. The column employed was a 10 μm LiChrosorb RP-18(250 mm × 4 mm),the elutingsolvent consisted of methanol—water—formic acid(85∶15 ∶ 0.8 v/v)and the effluent wasmonitored at 330 nm. The method is simple,rapid and accurate,the relative standard deviations ofthe three compounds are 0.996%,1.76%and 0.332%,respectively.