Abstract: AIM　To establish new methods for the chiral sepatation of three new drugs, ie, alfuzosin, terazosin and doxazosin. METHODS　By optimizing factors which affect the chiral separation, the kinds and concentrations of cyclodextrin, the pH value and the concentration of buffer, the optimum conditions for chiral separation were selected. RESULTS　The enantiomers of three new drugs were base-line-separated. CONCLUSION　The newly established method can be used to study the enantiomers of three new drugs.