三丁基磷酸酯化学修饰电极伏安法测定黄嘌呤

DETERMINATION OF XANTHINE BY VOLTAMMETRY WITH TRIBUTYL PHOSPHATE CHEMICALLY MODIFIED ELECTRODE

摘要: 目的：用三丁基磷酸酯化学修饰电极伏安法研究痕量黄嘌呤的测定。方法：在pH 9.5的NH₄OH—NH₄Cl缓冲溶液中,黄嘌呤在该化学修饰电极上于0.50 V(vs.SCE)左右产生一灵敏的氧化峰。结果：化学修饰膜选择性优先富集黄嘌呤，峰电流与浓度2.63×10^-7～7.89×10^-4 mol.L^-1呈线性关系,检出限为6.57×10-8 mol.L^-1 。结论：本法适用于血清、人尿中痕量黄嘌呤的测定。

Abstract: AIM: To study a quantitative method for determination of trace amounts of xanthine by voltammetry with a tributyl phosphate (TBP) modified glassy carbon electrode. METHODS: In a medium of NH₄OH-NH₄Cl buffer, a well defined anodic peak was observed at 0.50 V (vs.SCE). RESULTS: The chemically modified film preferentially accumulates the xanthine. The relationship between peak current and the concentration of xanthine was linear in the range of 2.63×10^-7～7.89×10^-4 mol.L-1. The detection limit has been estimated as 6.57×10^-8 mol.L^-1. CONCLUSION: This method has been shown to be suitable for the determination of xanthine in human serum, urine and potato samples with satisfactory results.