马双刚, 姜永涛, 宋少江, 王振华, 白景, 徐绥绪, 刘珂. 西洋参茎叶总皂苷碱降解成分西洋参茎叶总皂苷碱降解成分J. 药学学报, 2005, 40(10): 924-930.
引用本文: 马双刚, 姜永涛, 宋少江, 王振华, 白景, 徐绥绪, 刘珂. 西洋参茎叶总皂苷碱降解成分西洋参茎叶总皂苷碱降解成分J. 药学学报, 2005, 40(10): 924-930.
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MA Shuang-gang JIANG Yong-tao, SONG Shao-jiang, WANG Zhen-hua, BAI Jing, XU Sui-xu, LIU Ke, . Alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefoliumJ. Acta Pharmaceutica Sinica, 2005, 40(10): 924-930.
Citation: MA Shuang-gang JIANG Yong-tao, SONG Shao-jiang, WANG Zhen-hua, BAI Jing, XU Sui-xu, LIU Ke, . Alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefoliumJ. Acta Pharmaceutica Sinica, 2005, 40(10): 924-930.
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西洋参茎叶总皂苷碱降解成分西洋参茎叶总皂苷碱降解成分

Alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefolium

    摘要
  • 摘要: 目的研究西洋参茎叶总皂苷碱降解成分。方法采用硅胶柱色谱并结合HPLC进行分离纯化，通过波谱分析鉴定化合物的结构。结果从西洋参茎叶总皂苷碱降解产物中分离得到9种成分，分别鉴定为：20(S)-原人参二醇(I)，20(S)-达玛-25(26)-烯-3β,12β,20-三醇(II)，24(R)-ocotillol (III)，20(S)-原人参三醇(IV)，20(S)-达玛-25(26)-烯-3β,6α,12β,20-四醇(V)，达玛-20(21),24-二烯-3β,12β-二醇(VI)，达玛-20(21),24-二烯-3β,6α,12β-三醇(VII)，20(S),24(S)-达玛-25(26)-烯-3β,6α,12β,20,24-五醇(VIII)，20(S)-达玛-23-烯-25-过氧羟基-3β,6α,12β,20-四醇(IX)。结论碱降解20位S构型未改变。V，VII，VIII，IX为4个新化合物，并利用2D-NMR技术对新化合物的氢和碳的化学位移进行了归属。其中I对HCT-8人结肠癌细胞具有较强的细胞毒活性。

     

    Abstract: AimTo study the alkaline-degradation products of ginsenosides from leaves and stems of Panax quinquefolium L. MethodsIsolation and purification were carried out on silica gel and HPLC; the structures of chemical constituents were elucidated by spectral analysis. ResultsFrom the alkaline-degradation products, nine compounds were identified as: 20 (S)-protopanaxadiol (I), 20(S)-dammar-25(26)-ene-3β,12β,20-triol (II), 24(R)-ocotillol (III), 20(S)-protopanaxatriol (IV), 20(S)-dammar-25(26)-ene-3β,6α,12β,20-tetrol (V), dammar-20(21),24-diene-3β,12β-diol (VI), dammar-20(21),24-diene-3β,6α,12β-triol (VII), 20(S),24(S)-dammar-25(26)-ene-3β,6α,12β,20,24-pentanol (VIII), 20(S)-dammar-23-ene-25-hydroperoxyl-3β,6α,12β,20-tetrol (IX). Conclusion The configuration of C20 position of ginsenosides was not changed by alkaline-degradation. The complete assignments of 1H and 13C NMR chemical shifts of four new compounds V, VII, VIII, IX, were acquired by means of 2D NMR spectra. Compound I showed antitumor effect on human colon carcinoma cells in vivtro.

     

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