HPLC-ELSD法测定贝母中异甾类生物碱及糖苷类成分的含量

李萍; 曾令杰; 李松林; 毕志明; 林鸽

HPLC-ELSD法测定贝母中异甾类生物碱及糖苷类成分的含量

Determination of isosteroidal alkaloids and glucosides in Fritillaria by HPLC-ELSDSimultaneous determination of the major isosteroidal alkaloids and their glucosides in the bulbs of Fritillaria by high performance liquid chromatography coupl

摘要

摘要: 目的建立同时测定贝母中5种异甾类生物碱——peimissine, imperialine, sinpeinine A, imperialine-3β-glucoside和yibeinoside A含量的HPLC分析方法。方法C₁₈柱;流动相:乙腈-水(含0.1%二乙胺);梯度洗脱,流速1.0 mL·min^-1;检测器:Alltech 500蒸发光散射检测器(ELSD)。结果线性范围为peimissine 13.1～288.2 mg·L^-1(r²=0.997 5), imperialine-3β-glucoside 7.7～169.4 mg·L^-1 (r²=0.999 3), yibeinoside A 7.3～160.6 mg·L^-1 (r²=0.999 7), imperialine 16.5～363.0 mg·L^-1 (r²=0.999 2), sinpeinine A 8.7～191.4 mg·L^-1 (r²=0.994 2)。 5个化合物的精密度和重现性RSD均<5%。结论本方法简便、有效、可行,可用于贝母中5种异甾类生物碱的含量测定。

Abstract: AimTo establish an HPLC-ELSD method for the simultaneous determination of five major bioactive isosteroidal alkaloids and gluco-alkaloids in the bulbs of Fritillaria namely peimissine, imperialine, sinpeinine　A, imperialine-3β-glucoside and yibeinoside A. MethodsA Nova-Pak C₁₈ column (150 mm×3.9 mm ID) was used. The chromatography was carried out with a linear gradient programming. The mobile phase was acetonitrile-water (containing 0.1% diethylamine) and the flow rate was 1.0 mL·min^-1. ResultsThe linear range of peimissine was 13.1-288.2 mg·L^-1 (r²=0.997 5), imperialine-3β-glucoside 7.7-169.4 mg·L^-1 (r²=0.999 3), yibeinoside A 7.3-160.6 mg·L^-1 (r²=0.999　7), imperialine 16.5-363.0 mg·L^-1 (r²=0.999　2), sinpeinine A 8.7-191.4 mg·L^-1 (r²=0.994　2). ConclusionThe method is accurate with overall intra- and inter-day variation less than 5% and recovery more than 95%. The method was successfully applied to analyze five major bioactive alkaloids and gluco-alkaloids in three Fritillaria bulbs.

HTML全文

参考文献(0)

施引文献

资源附件(0)