大蒜中两种新的甾体皂甙成分及其对血液凝聚性的影响

TWO NEW STEROIDAL SAPONINS FROM ALLIUM SATIVUM AND THEIR INHIBITORY EFFECTS ON BLOOD COAGULABILITY

摘要: 用大孔树脂(AB-8)柱层析、硅胶柱层析、Sephadex LH-20柱层析、及制备性HPLC等方法,从大蒜(Alium sativum L.)水溶性部分分到6个化合物,并鉴定其结构,它们分别是proto-iso-eruboside-B(I),eruboside-B(I),iso-eruboside-B(II),sativoside-C(IV),腺苷(V)和色氨酸(VI)。其中I和II为两个新的甾体皂甙类化合物。并测试了它们对血液系统的作用,发现大蒜总甙有抗血小板聚集和提高纤溶活性,iso-eruboside-B有明显的延长血液凝固时间和提高纤溶活性,proto-iso-eruboside-B有显著的提高纤溶活性,腺苷则有抗血小板聚集活性和提高纤溶活性,sativoside-C由于量少未测活性。其余化合物均无活性。

Abstract: Six compunds were isolated from the fresh bulbs of Allium sativum L(garlic). Their structures were elucidated as proto-iso-eruboside-B (I), eruboside-B (II), iso-eruboside-B (III), sativioside-C (IV), adenosine (V) and tryptophan (VI). I and III are new steroidal saponins. This paper deals with the structural determination of I and III and their effects on platelet aggregation, blood coagulation and fibrinolysis in vitro .