Abstract: The local injection of trypsin can be used in clinical practice as a new and effective therapy for snakebite.Our experiments indicate that when a lethal dose of crotalinae snake venom was injected into mice subcutaneously, survival rate increased significantly if a dose of trypin had been injected locally and promptly.When lethal doses of the venoms (Trimeresurus 700 μg, Agkistrodon acutus 450 μg, A. halys 180μg) were injected into mice and trypsin is given, the survival rate is 68%, 57%, 67%.We've treated 207 cases of snakebite without death. The oroduction of trypsin is rather simple and its preservation needs no special condition. Trypsin can be put into portable first-aid auto-injector and can be used after snakebite at the earliest possible time.