基于溶解氧乙酰螺旋霉素极谱平行催化氢波的研究

Parallel catalytic hydrogen wave of acetylspiramycin caused by dissolved oxygen and its application

摘要: 目的提出一种极谱测定乙酰螺旋霉素(ASPM)的新方法。方法应用单扫描极谱法，在含溶解氧的0.1 mol·L^-1 NH₄Cl-NH₃·H₂O(pH 8.9)缓冲液中ASPM产生1个灵敏的平行催化氢波［峰电位E_p=-1.63 V(vs SCE)］。结果该平行催化氢波的二阶导数峰峰电流i″与ASPM浓度在1.74×10^-3～3.48 μg·mL^-1呈良好线性关系(r=0.997 9,n=13)，检出限为5.80×10^-4 μg·mL^-1(3σ)。对0.871 μg·mL^-1 ASPM溶液进行13次平行测定，RSD为1.24%。结论本方法可用于ASPM片剂中ASPM含量的测定。

Abstract: AimTo propose a novel polarographic method for the determination of acetylspiramycin (ASPM) is proposed. MethodsIn 0.1 mol·L^-1 NH₄Cl-NH₃·H₂O (pH 8.9) buffer containing dissolved oxygen, ASPM yielded a sensitive parallel catalytic hydrogen wave with the peak potential of -1.63 V (vs SCE) by single sweep polarography. ResultsThe 2nd order derivative peak currents (i_p″) of the parallel catalytic hydrogen waves of ASPM showed a linear relationship with its concentrations in the range from 1.74×10^-3 μg·mL^-1 to 3.84 μg·mL^-1 (r=0.997 9, n=13). Its detection limit was 5.80×10^-4 μg·mL^-1 (3σ) and RSD (n=13) was 1.24% at the concentration level of 0.871 μg·mL^-1. ConclusionThe proposed method could be applied to the determination of ASPM in ASPM tablets.