葛根素的高效液相色谱分离和含量测定

THE SEPARATION AND DETERMINATION OF PUERARIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

摘要: 本文用反相高效液体色谱法对葛根素进行分离和含量测定。色谱柱为partisil-10μmODS,流动相为MeOH-H₂O-CHCl₈系统,葛根总黄酮在40分钟内可以完全分离。以外标法,在254nm检测对葛根素进行含量测定。本文还报道了利用C₁₀反相制备型液体色谱柱对葛根素和与它难分离的另一组分(暂名OMe葛根素)的制备分离。

Abstract: This paper reports the separation and quantitative determination of puerarin by HPLC method. The column used was Partisil-10μODS with MeOH:H₂O:CHCl₃ system as the mobile phase. Total flavonoid of pueraria can be separated within 40 minutes. The content of puerarin was determined at 254 nm by external standard method.Preparation and isolation by C₁₆ reverse phase column of a constituent(tentatively named OMe-puerarin) which is separated from puerarin only with much difficulty is also described.