牛膝甾酮25位差向异构体的分离与鉴定

Isolation and structure identification of C-25 epimers of inokosterone from Achyranthes bidentata Blume.

摘要: 目的分离并确定苋科植物怀牛膝(Achyranthes bidentata Blume.)中牛膝甾酮25位差向异构体的结构。方法利用色谱技术分离纯化牛膝甾酮25位差向异构体，用波谱(IR,UV,MS,NMR)方法及化学方法确定结构。结果从怀牛膝的乙酸乙酯部位分离得到3个化合物，分别鉴定为25S-牛膝甾酮(1, 25S-inokosterone)，25R-牛膝甾酮(2, 25R-inokosterone)，β-蜕皮甾酮(3, ecdysterone)。结论化合物1和2为首次从怀牛膝中分离得到的25位差向异构体，首次确定了25位绝对构型和发表该25位差向异构体的¹³CNMR数据。

Abstract: AimTo isolate C-25 epimers of inokosterone from Achyranthes bidentata Blume. and identify their structures. MethodsTo separate C-25 epimers of inokosterone by using various kinds of chromatography methods and identify their structures on basis of spectral analysis and chemical method. ResultsThree compounds were isolated and their structures were established as 25S-inokosterone (1), 25R-inokosterone (2) and ecdysterone (3). ConclusionCompounds 1 and 2 are new C-25 configuration isomers from Achyranthes bidentata Blume., their absolute configurations are elucidated at the first time, and their ¹³CNMR data are reported for the first time.