Abstract: AIM：To study the effects of volatile sulfur compounds isolated from caucas (Allium victorialis L.) on spontaneous beating of cultured mouse myocardial cells. METHODS: Cultured mouse myocardial cells were prepared. The effects of five compounds from caucas on the rate and amplitude of beating of cultured myocardial cells were examined by using microcomputer-driven image analyzing system. RESULTS: Of these compounds, dimethyl disulfide(DMDS), methyl allyl trisulfide(MATS), methyl allyl sulfide(MAS) and diallyl disulfide(DADS) appreciably increased the beating rate and beating amplitude, and significantly inhibited Ca²⁺ ATPase activity, but non-sulfur 2-pentanone did not affect them. CONCLUSION: The positive inotropic effect on cultured myocardial cell sheets induced by sulfur compounds may be correlated with its inhibition on enzyme.