蔡金娜, 周开亚, 徐珞珊, 王峥涛, 沈曦, 王义权, 李晓波. 中国不同地区蛇床的rDNA ITS序列分析J. 药学学报, 2000, 35(1): 56-59.
引用本文: 蔡金娜, 周开亚, 徐珞珊, 王峥涛, 沈曦, 王义权, 李晓波. 中国不同地区蛇床的rDNA ITS序列分析J. 药学学报, 2000, 35(1): 56-59.
shu
Wang Yiquan, Cai Jinna, Zhou Kaiya, Xu Luoshan, Wang Zhengtao, Shen Xi, , Li Xiaobo. RIBOSOMAL DNA ITS SEQUENCE ANALYSES OF CNIDIUM MONNIERI FROM DIFFERENT GEOGRAPHICAL ORIGIN IN CHINAJ. Acta Pharmaceutica Sinica, 2000, 35(1): 56-59.
Citation: Wang Yiquan, Cai Jinna, Zhou Kaiya, Xu Luoshan, Wang Zhengtao, Shen Xi, , Li Xiaobo. RIBOSOMAL DNA ITS SEQUENCE ANALYSES OF CNIDIUM MONNIERI FROM DIFFERENT GEOGRAPHICAL ORIGIN IN CHINAJ. Acta Pharmaceutica Sinica, 2000, 35(1): 56-59.
shu

中国不同地区蛇床的rDNA ITS序列分析

RIBOSOMAL DNA ITS SEQUENCE ANALYSES OF CNIDIUM MONNIERI FROM DIFFERENT GEOGRAPHICAL ORIGIN IN CHINA

    摘要
  • 摘要: 目的：探讨不同分布区的蛇床Cnidium monnieri的ITS序列变异与其地理分布和化学成分的相关性。方法：设计2对引物，Pf+Pb及P5.8S ITS1+P5.8S ITS2，PCR扩增产物纯化后用银染法或ABI 310测序。结果：得到核糖体DNA中的ITS及5.8S rDNA完全序列，18S和26S rDNA部分序列,共约700 bp。5个地点样品的ITS-1及ITS-2的序列大小分别为210～217 bp和219～224 bp。ITS-1碱基序列的遗传距离0.00～1.93%，ITS-2碱基序列的遗传距离0.46～2.34%，ITS-1较为保守。以NJ法根据ITS-2序列数据重建系统发生树。哈尔滨样品聚为一组，衡水与德州样品和郑州与高淳样品各自聚为一组。结论：ITS-2序列的变异与中国产蛇床的纬度分布相关，而其与蛇床化学型的关系尚需作进一步研究。

     

    Abstract: AIM：To study the correlation between ITS sequence variation of Cnidium monnieri and geographical distribution and chemo-type of the plant. METHODS: Two pairs of primers Pf+Pb and P5.8S ITS1+P5.8S ITS2 were designed. The PCR products were purified and sequenced by silver staining DNA sequencing technique and ABI 310. RESULTS: Complete sequcnce of ITS and 5.8S rDNA, and partial sequence of 18S rDNA and 26S rDNA of Cnidium monnieri were obtained. They totaled about 700 bp. The sequences of ITS-1 and ITS-2 ranged from 210 bp to 217 bp and 219 bp to 224 bp; respectively. Pairwese sequence divergences of the samples ranged from 0% to 1.93% in ITS-1, and from 0.46% to 2.34% in ITS-2. The ITS-1 is more conservative. Phylogenetic tree based on ITS-2 sequence data (225 aligned sites) indicated that the Harbin samples, Hengshui and Dezhou samples, and Zhengzhou and Gaochun samples clustered separately. CONCLUSION: The sequence variation of ITS-2 is correlated to latitude distribution of Cnidium monnieri in China. Further studies are needed to clarify the relation between ITS sequence variation and chemo-type of Cnidium monnieri.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回