Abstract: AimTo find corresponding relationship between the fingerprint of Houttuynia cordatainjections from different factories and their effects. MethodsHouttuynia cordata injections from six different factories were determined by gas chromatography (GC) and gas chromatography-mass spectra (GC-MS), and GC fingerprints were classified by hierarchical clustering. The anti-inflammatory activity of Houttuynia cordata injections was characterized through the rat pleurisy model induced by carrageenin and the mice ear edema model by dimethylbenzene. ResultsThe anti-inflammatory effect of the injections from the first class factories on the two model was significant, while those from the second class not. GC-MS analysis result indicated that main effect compounds in Houttuynia cordata injections are methyl n-nonyl ketone, decanoylacetaldehyde, lauryl aldehyde, capryl aldehyde, β-pinene, β-linalool, 1-nonanol, 4-terpineol, α-terpineol, bornyl acetate, n-decanoic acid and acetic acid, geraniol ester etc. ConclusionThere is corresponding relationship between the fingerprint of Houttuynia cordata injections and effect to a certain extent.