Abstract: The polarographic behavior of gossypol in various buffers was studied. It was found that below pH 5, a well-defined reduction wave was obtained. When pH was higher than 5, the wave was deteriorated and could not be measured accurately. According to studies on the effect of mercury height and temperature on the limiting current, the relationship between E and lg(i/i_d-i), the dependence of the half-wave potential on pH and the influence of temperature and gossypol concmtration on half-wave potential, it was concluded that this wave is diffusion-controlled and corresponds to an 1-electron reversible reduction of one aldehyde group of gossypol.For quantitative analysis, it is recommended to use a medium containing 60% acetone and a pH 2.6 solution of 0.1 M citric acid. The half-wave potential of gossypol under this condition is -1.14 V vs SCE. This condition can be used in, pulse polarography.