白三烯类化合物(Leukotrienes)对小鼠腹腔巨噬细胞生成白细胞介素6的影响

EFFECTS OF LEUKOTRIENES ON PRODUCTION OF INTERLEUKIN 6 FROM MOUSE PERITONEAL MACROPHAGES

摘要: 探讨了白三烯B₄(LTB₄)、白三烯C₄(LTC₄)及白三烯D₄(LTD₄)对小鼠腹腔巨噬细胞分泌白细胞介素6(interleukin6,IL-6)的影响。用IL-6依赖细胞株B9的MTT方法测定样品中IL-6的含量。结果显示,LTB₄能剂量依赖性地增加巨噬细胞培养上清液中IL-6的含量。LTC₄及LTD₄促进巨噬细胞培养上清液中IL-6含量的最适浓度分别为:6.9×10^-8和8.05×10^-8mol·L^-1。结果提示:LTB₄与LTC₄及LTD₄在某些生物学功能方面有一致性。与LTC₄及LTD₄相比,LTB₄促进巨噬细胞培养上清液中IL-6的含量的最适浓度则要大1～2个数量级,提示在炎症反应中,肽白三烯与IL-6的相关性较强。

Abstract: In the present study, effects of leukotrienes on IL-6 production by mouse resident peritoneal macrophages were explored with a bioassay method involving IL-6 dependent murine hybridoma B9 cell line. The results showed that LTB₄, LTC₄ and LTD₄ can enhance the production of IL-6 from cultured peritoneal macrophages of mice. The optimal concentractions for LTB₄, LTC₄ and LTD₄ were found to be 1.45×10^-6, 6.9×10^-8 and 8.05×10^-8 mol·L^-1, respectively. The results suggest that peptide leukotrienes might play important role on IL-6 production in the local milieu of inflammation.