Abstract: In this paper, a method——improved emulsification vesicles (IEV) was proposed for the preparation of adenosine triphosphate (ATP) liposome The-ATP entrapment ratio reached 38.9% (w/w), which was much greater than that reported in the literature. Thus, IEV is an excellent method which has never been presented. Together with ultrafiltration for removing unentrapped drugs and radiation sterilization, these methods are most meaningful for the production of liposomes in large scale.