Abstract: From the overground part of Sophora algpecuroides L eight alkaloids were isolated. On the basis of m p, element analysis and spectrometric analysis these alkaloids were identified as Sophocarpine (yield 0.08%), Matrine (yield 0.06%), Sophoramine (yield 0.02%), Sophoridine (yield 0.20%), Aloperine (yield 0.12%), N-Methylcytisine (yield 0.001%), Cytisine (yield 0.007%) and Oxymatrine (yield 0.006%). Among them N-Methylcytisine was isolated from this plant for the first time.