半枝莲多糖B3-PS2分离纯化及抗补体活性研究

Anti-complement activity of polysaccharide B3-PS2 purified from Herba Scutellariae Barbatae

摘要:
本文提取半枝莲多糖，分别经DEAE-cellulose、Sephacryl S-300柱层析分离纯化后得到均一的B3-PS2组分，并对该组分进行了抗补体活性研究。研究结果表明B3-PS2组分分子质量为1 100 kD，主要由Glc、Gal和Ara三种单糖组成，糖残基摩尔比为2.7∶2.7∶1.0，还含有少量的Man、Rha、Fuc和Xyl。B3-PS2抑制补体活性的经典途径CH₅₀为(0.23 ± 0.03) mg·mL⁻¹，抗补体作用靶点为补体C1r、C1s、C3、C4组分。半枝莲多糖B3-PS2组分体外抗补体活性接近于阳性对照肝素，表明活性较好，这显示了半枝莲多糖治疗补体过度激活相关疾病的潜在价值。

Abstract:
The polysaccharide B3-PS2 was extracted and purified from Herba Scutellariae Barbatae through chromatography of DEAE-cellulose and Sephacryl S-300 column. Average molecular weight of B3-PS2 was about 1 100 kD. It was composed of Glc, Gal and Ara in the ratio of 2.7∶2.7∶1.0, along with trace of Man, Rha, Fuc and Xyl. B3-PS2 inhibited complement activation on the classic pathways with CH₅₀ value of (0.23 ± 0.03) mg·mL⁻¹. The targets of B3-PS2 upon the complement system were C1r, C1s, C3 and C4. These results suggested that anti-complementary activity of B3-PS2 was closed to its positive control heparin. It strongly suggested that the polysaccharide B3-PS2 from Herba Scutellariae Barbatae could be a potential candidate in treating those complement-associated diseases.