This study was to comprehensively evaluate the chemical quality of main species of epimedium planted in China.  The contents of 5 marker compounds, epimedin A, epimedin B, epimedin C, icariin and baohuoside I, as well as total flavonoids of 22 samples of 8 officinal species of Epimedium were determined by HPLC and UV, separately.  Some physical and chemical tests (H₂O, total ash, acid-insoluble ash and EtOH extract) were also carried out to investigate their chemical qualities.  There were significant differences in types and contents of prenyl-flavonoid glycosides such as epimedin A, epimedin B, epimedin C, icariin and baohuoside I in different species, meanwhile, the physical and chemical parameters results also showed that there were obvious differences in chemical quality among different species of epimedium herb.  The results provide theoretical and experimental basis for the establishment of comprehensive quality assessment system of epimedium in China.