Abstract: AimTo study the lignan glycosides from the roots of Bupleurum scorzonerifolium.MethodsChromatography methods was used to isolate compounds and chemical and spectral methods were used to identify the structures of isolated compounds.ResultsTwo lignan glycosides were isolated from the roots of B.scorzonerifolium and their structures were identified as 2,3-E-2,3-dihydro-2-(3′-methoxy-4′-O-β-D-glucopyranosyl-phenyl)-3-hydroxymethyl-5-(3″-hydroxypropenyl)-7-methoxy-1-benzo［b］furan (1) and 2,3-E-2,3-dihydro-2-(3′-methoxy-4′-hydroxy-phenyl)-3-hydroxymethyl-5-(3″-hydroxypropenyl)-7-O-β-D-glucopyranosyl-1-benzo［b］furan (2), both of them which were the mixture of (+)2S,3R- and (-)2R,3S-type diastereoisomer and in both of them the amount of (+)2S,3R- type was a little more than that of the (-)2R,3S-type.ConclusionCompounds 1 and 2 were isolated from B.scorzonerifolium for the first time.(+)2S,3R-2, 3-Dihydro-2-(3′-methoxy-4′-hydroxy-phenyl)-3-hydroxymethyl-5-(3″-hydroxypropenyl)-7-O-β-D-glucopyranosyl-1-benzo［b］furan is a new compound.