Synthesis, biological activity and molecular docking research of N-(4-oxo-thiochroman-3-yl)phenyl-methylacetamide derivatives as α-glucosidase inhibitors

In order to develop potent antidiabetic agents that have inhibitory effect to α-glucosidase, twelve β-acetamido ketone derivatives such as N-(substituted-4-oxo-thiochroman-3-yl)phenyl-methylacetamide are designed and synthesized through one-pot Dakin-West reaction. Their chemical structures are confirmed by ¹H NMR, ¹³C NMR, IR and HR-MS. In vitro α-glucosidase inhibition assays of compounds 4a-4l were carried out using glucose oxidase method. The result indicated that most of them possess inhibitory activity in vitro. Compound 4k showed the most potent inhibitory activity with 87.3% inhibition of α-glucosidase at the concentration of 5.39 mmol·L^-1. The structure-activity relationship of these β-acetamido ketone derivatives was discussed preliminarily. Moreover, the molecular docking method was used to study the interaction mode of compound 4k and α-glucosidase. Our results will be helpful for designing of α-glucosidase inhibitors in the future.