A new patchoulane-type sesquiterpenoid from patchouli oil and its anti-inflammatory activity

A new 7, 10∶1, 5-patchoulane-type sesquiterpenoid was isolated and purified by silica gel column chromatography, medium pressure liquid chromatography, and semi-preparative high performance liquid chromatography. Based on IR, HR-ESI-MS, NMR, and X-single crystal diffraction data analyses, compound 1 was determined to be (-)-(3S, 4R, 5R, 7R, 10R)-7, 10∶1, 5patchoul-1(2)-en-3, 4-diol. It showed an inhibitory effect on LPS-induced NO production in RAW264.7 cells.