HE Li-dan, ZHAO Kai-jun, PENG Yun, HUANG Fang, WANG Hai-li. DNA molecular identification of Polyrhachis dives medicinal materialsJ. Acta Pharmaceutica Sinica, 2023, 58(10): 3140-3146. DOI: 10.16438/j.0513-4870.2023-0269
Citation: HE Li-dan, ZHAO Kai-jun, PENG Yun, HUANG Fang, WANG Hai-li. DNA molecular identification of Polyrhachis dives medicinal materialsJ. Acta Pharmaceutica Sinica, 2023, 58(10): 3140-3146. DOI: 10.16438/j.0513-4870.2023-0269

DNA molecular identification of Polyrhachis dives medicinal materials

  • In the study, specific primers were designed based on the CO Ⅰ gene sequence of Polyrhachis dives. By optimizing the genomic DNA extraction method and amplification conditions, we established an efficient, specific, and accurate DNA molecular identification method for Polyrhachis dives. In this method, the length of the target fragment was 294-308 bp, and the other counterfeits had no target bands. In this paper, the specific identification method of the origin of Polyrhachis dives established can be used to identify the medicinal materials of Polyrhachis dives accurately.
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