Studies on the chemical composition of Ferula feruloides

Eleven compounds were isolated and purified from the ethyl acetate part of 80% ethanol extract of Ferula feruloides root by a combination of normal-phase silica gel column chromatography, Sephadex LH-20 dextran gel column chromatography and semi-preparative liquid chromatography and then modern wave spectrometry methods (NMR, MS, UV, IR) were used to identify the structures of the compounds, which were identified as baigene D (1), baigene E (2), baigene F (3), β-kirialovin (4), α-kirialovin (5), falcarindiol (6), ammoresinol (7), dshamirone (8), 2, 3-dihydro-7-hydroxy-2S^*, 3R^*-dimethyl-3-4-methyl-5-(4-methy1-2-furyl)-3(E)-pentenyl-furo3, 2-ccoumarin (9), 2, 3-dihydro-7-hydroxy-2S^*, 3R^*-dimethyl-2-4, 8-dimethyl-3(E), 7-nonadi-enyl-furo3, 2-ccoumarin (10), and baigene C (11). Compounds 1-3 are new coumarin analogues, and compounds 4-6 are firstly isolated from F. feruloides. The anti-proliferative activity of compounds 5, 7-11 against human gastric cancer (MKN-45) cells was evaluated using MTT assay, which showed that compounds 7-11 exhibited strong inhibitory activity, and compound 5 exhibited weak inhibitory activity.