Design, synthesis and activity evaluation of novel FLT3 inhibitors

In this study, a series of 4-phenoxypyridine derivatives containing imidazole-4-formamido and 1,2,4-triazole were designed and synthesized as novel inhibitors of Fms-like tyrosine kinase 3 (FLT3). The results showed that the series of compounds had good antiproliferative activity. Among them, the preferred compound 10c was in two cell lines containing FLT3-ITD (internal tandem duplication, ITD) mutations, namely human myeloid core solo leukemia cells (MV4-11) and human acute myeloid leukemia cells (Molm-13), with IC₅₀ values of 1.90 and 5.78 μmol·L^-1, respectively. Apoptosis and cycle results showed that compound 10c could promote apoptosis and prevent cells in S phase. Molecular docking results showed that compound 10c formed two π-π stacking effects with PHE691 and HIS809 of FLT3, and the pyridine parent nucleus and Linker part formed important hydrogen bonds with LYS644 and CYS694 of FLT3, respectively. In summary, compound 10c exhibited good anti-proliferation activity in vitro, which is of value for further in-depth research, and also lays the foundation for the development of more novel FLT3 inhibitors with novel structures.