DIFFERENTIAL REGULATION OF DOPAMINE PECEPTORS ON PRE-AND POSTSYNAPTIC Na⁺,K⁺-ATPase IN PAT STRIATUM

The pre and postsynaptic membranes isolated from rat striatum were used to investigate the regulation of dopamine receptors on striatal Na⁺, K⁺ ATPase in these membranes. The activity of Na⁺, K⁺-ATPase was determined by colorimetric method. Dopamine (DA) was found to inhibit the Na⁺, K⁺ ATPase activity on postsynaptic membranes in a concentration dependent manner with a IC₅₀ value of 4.6μmol·L^-1. This inhibitory effect was reversed by either selective D1 receptor antagonist SCH23390 or selective D2 receptor antagonist spipernone. The inhibitory effect similar to DA was produced by combination with selective D1 receptor agonist SKF38393 and selective D2 receptor agonist LY171555. In contrast, under the same experimental conditions, DA (10^-8～10^-5 mol·L^-1) was shown to activate the activity of Na⁺, K⁺-ATPase on presynaptic membranes in a concentration dependent manner. Meanwhile, the stimulatory effect was reversed by spiperone alone rather than by SCH23390. These results show the differential regulation of presytnaptic and postsynaptic DA receptors on Na⁺,K⁺-ATPase in rat striatum.