STUDIES ON THE SAPONINS FROM THE ROOT OF GLYCYRRHIZA URALENSIS FISCH

This paper reports the isolation and determination of three triterpene glucosides named uralsaponin A(Ⅰ), uralsaponin B(Ⅱ) and glycyrrhizin (Ⅲ) from the root of Glycyrrhiza uralensis Fisch. Their chemical structures were elucidated by means of chemical and spectrometric analysis (UV, IR, MS, ¹H-HMR and ¹³CNMR).Uralsaponin A(Ⅰ), C₄₂H₆₂O₁₆, was obtained as granular white crystals, mp235℃ (d.), α_D¹⁸+42.7(c,0.45,90%MeOH),Its structure was found to be 3β-hydroxy-11-oxo-olean-12-en-30-oic acid-3-0-β-D-glucuronopyranosyl- (1-2)-β-D-glucuronopyranoside(Ⅰ).Uraisaponin B(Ⅱ),C₄₂H₆₂O₁₆,was obtained as white, granular white crystals, mp 244℃ (d.),(d.), α_D¹⁸+31.0(c,0.29,90%MeOH), Its structure was found to be 3β-hydroxy-11-oxo-olean-12-en-30-oic acid-3-0-β-D-glucuronopyranosyl-(1-3)-β-D-glucuronopyranoside(Ⅱ).Uralsaponin A(Ⅰ) and Uralsaponin B(Ⅱ) are all new triterpenoid saponins. The known saponin was identified to be 3β-hydroxy-11-oxo-olean-12-en- 30-oic acid-3-β-D-glucuronopyranosyl- (1-2) -α-D-glucuronopyranoside, Glycyrrhizin (Ⅲ).