Simultaneous determination of three kinds of components in Sanhuang tablets by high-performance liquid chromatography

AimTo establish an assay method for the determination of three kinds of biologically active components, five compounds (emodin, chrysophanol, baicalin, wogonin and berberine hydrochloride) simultaneously in Sanhuang tablets. MethodsHPLC was carried out, using a C₁₈ column (150 mm×4.6 mm ID, 5 μm) set at 30 ℃, acetonitrile-0.02 mol·L^-1 acetic ammonium (adjusted pH to 3.50 with acetic acid glacial) as mobile phase (using gradient) with flowing rate 1.00 mL·min^-1 and detected at 270 nm. ResultsThe calibration curve of emodin was linear from 0.020 7 μg to 0.207 μg with r=0.999 9, the average recovery was 99.65% with RSD 1.25%. The calibration curve of chrysophanol was linear from 0.052 μg to 0.52 μg with r=0.999 9, and the average recovery was 100.36% with RSD 0.96%. The calibration curve of baicalin was linear from 0.250 5 μg to 2.505 μg with r=0.999 8, and the average recovery was 100.22% with RSD 1.29%. The calibration curve of wogonin was linear from 0.047 6 μg to 0.476 μg with r=0.999 9, and the average recovery was 98.97% with RSD 1.20%. The calibration curve of berberine hydrochloride was linear from 0.053 12 μg to 0.531 2 μg with r=0.999 5, and the average recovery was 96.02% with RSD 2.02%. The established method had also been used in the determination of the 5 compounds in 10 different batches of Sanhuang tablets. ConclusionThis method was proved to be accurate and quick, and can be used for the quality control of the preparation all-around.