The protective effects of ginsenoside Rg1 and Rb1 against damage of HaCaT cells by ultraviolet B

AimTo investigate the survival rate and the level of HaCaT cells damage with ultraviolet B (UVB) radiation at various doses, and observe the protective effects of ginsenoside Rg1 and Rb1in vitro. MethodsMTT assay was employed to analyze the cell survival rate after UVB radiation of 30, 60, 90 and 120 mJ·cm^-2. The damage of nucleolus and the protective effects of ginsenoside Rg1 and Rb1 were scanned by Hoechst 33258 staining and single cell gel electrophoresis assay (SCGE). ResultsIt was found that the cell survival rate decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ·cm^-2 to 120 mJ·cm^-2. At the dose of 20 μg·mL^-1, obvious protective effect of ginsenoside Rg1 and Rb1 can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage. ConclusionUVB radiation inhibits HaCaT human keratinocytes growth and ginsenoside Rg1 and Rb1 can relief the damage.