ANALYSIS OF PAEONOL IN PAEONIA SUFFRUTICOSA ANDR. AND ITS PATENT PREPARATIONS BY SUPERCRITICAL FLUID EXTRACTION AND CAPILLARY GAS CHROMATOGRAPHY

A supercritical fluid extraction (SFE) method was developed for the extraction of paeonol in Paeonia suffruticosa Andr. and its patent preparations. A systematic method was used to optimize five variables (temperature, pressure, modifier concentration, static extracting time and CO₂ dynamic extracting volume) of SFE. Sample extracts were analyzed by wide-bore capillary gas chromatography (CGC). The method showed good linearity (γ=0.9999)in the range of 0.1～1.6 mg·ml^-1 of paeonol. The average recovery was 97.8% with RSD=2.35% (n=3) for Paeonia suffruticosa andr., 100.3% with RSD=1.89% (n=3) for its patent preparations.