INHIBITORY EFFECTS OF THREE Gn COMPOUNDS ON TNF_α PRODUTION BY MURINE PERITONEAL MACROPHAGES

AIM　To investigate the effects of isorhapotigenin (Gn-1), resveratrol (Gn-2) and stilbene oligopolymer (Gn-3) on TNF_α production by murine peritoneal macrophages stimulated with LPS or rhIL-8. METHODS　Inhibition was used to show the inhibitory effect of three compounds on TNF_α production by murine peritoneal macrophages measured with crystal violet staining assay of L929 cells. RESULTS　Isorhapotigenin(10^-5～10^-4 mol.L^-1), resveratrol(10^-5～10-4 mol.L^-1) and stilbene oligopolymer(10^-6～10^-4 mol.L^-1） were shown to inhibit TNF_α production by murine peritoneal macrophages stimulated with LPS in a dose-dependent manner, IC₅₀ are 4.9×10^-5 mol.L^-1，5.2×10^-5 mol.L^-1 and 3.6×10-5 mol.L^-1 respectively. Isorhapotigenin（5×10^-6～10^-4 mol.L^-1） and stilbene oligopolymer(10^-6～10^-4 mol.L^-1） were also found to dose-dependently inhibit TNF_α production by murine peritoneal macrophages stimulated with rhIL-8, their IC₅₀ are 9.3×10^-5 mol.L^-1 and 4.6×10-6 mol.L^-1 respectively. CONCLUSION　The results suggest that the three Gn compounds can inhibit TNF_α production by murine peritoneal macrophages, which is probably one of the mechanisms of the anti-inflammatory effects of isorhapotigenin, resveratrol and stilbene oligopolymer.