THE PREPARATION OF SUBSTITUTED UREAS AND THEIR IN VITRO BACTERIOSTATIC ACTIVITY

A number of substituted ureas (Ⅳ) and related intermediates were prepared. Their methods of preparation and physical data were described and tabulated in Table 1. The in vitro bacteriostatic activity of these compounds was tested against Pseudomonas aerug-inosa (PA) and Micrococcus pyogenes var. aureus (MPA) and the results were sum- marized in Tables 2 and 3. Compound 18 (Ⅳ, R¹=Br, R²=(?),X=O), the most active against PA, inhibits the growth at a concentration of 8 micrograms per millilitre. Compound 1 (Ⅳ, R¹=Br, R²=(?)-Br, X=S), the most active against MPA, inhibits the growth at a concentration less than one microgram per millilitre.