DETERMINATION OF ANNONACEOUS ACETOGENINS IN ANNONACEAE PLANTS BY HPLC

AIM To develop a method for analysis of antitumor annonaceous acetogenins in Annonaceae plants by HPLC. METHODS Squamostatin-B (1), squamocin (2) and annonin-VI (3) were used as standard substances. Chromatography column was a Rp-18; the mobile phase was methanol-water (90∶10); the flow rate was 1.0 mL·min^-1 and the detecting wavelength was 220 nm. RESULTS A linear range was obtained from 2.3 to 13.8 μg with a good correlation. The recoveries of (1), (2) and (3) were 100.3%, 100.3% and 100.0%, respectively. CONCLUSION This method was developed for the analysis of acetogenins by HPLC for the first time. The method is rapid, accurate and suitable for the analysis of the antitumor acetogenins in Annonaceae plants.