Determination of peimine and peiminine in Fritillaria thunbergii by HPLC-ELSD

AimTo determine peimine and peiminine in Fritillaria drug simultaneously by RP-HPLC-ELSD. MethodsHPLC was carried out with a Waters Alliance, Model 2690,equipped with XTerra RP18 column (150 mm×3.9 mm ID, 5 μm) and evaporated light scattering detector. The mobile phase (acetonitrile-10 mmol·L^-1 NH₄HCO₃ adjusted to pH 10.10 by ammonia solution) was eluted in gradient mode. ResultsThe recoveries of peimine and peiminine were 98.96% (n=4), with RSD 1.01% and 98.40% (n=4), with RSD 2.63%, respectively. ConclusionThe method is simple, sensitive and reliable. It can be used for quantitative determination of Fritillaria drug.