Apigenin glycosides from Euphorbia humifusa Wild.

The investigation on the herbal of Euphorbia humifusa Wild. was carried out in order to find its anti-HBV constituents.  The isolation and purification were performed by chromatography such as Sephadex LH-20, MCI GEL CHP 20P, etc.  Based on the spectral analysis, five apigenin glycosides were identified as apigenin-7-O-(6"-O-galloyl)-β-D-glucopyranoside (1), apigenin-7-O-β-D-apiofuranosyl (1→2)-β-D-glucopyranoside (2), apigenin-7-O-β-D-lutinoside (3), apigenin-7-O-β-D-glucopyranside (4) and apigenin (5).  Among them, compound 1 is a new compound, compound 2 and 3 were isolated from this plant for the first time.