Protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline in vitro

AimTo investigate the cardio-protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline. MethodsAfter adding crocetin, the primary culture of cardiac myocyte was injured by 1.0 μmol·L^-1 noradrenaline. The activity of lactic dehydrogenase (LDH), mitochondrion succinic dehydrogenase (MSDH) and ATPase were assayed. The mitochondrion membrane potential was detected by Rh123. The percentage of cardiac myocyte apoptosis was observed by flow cytometry. ResultsCrocetin significantly decreased the activity of LDH in culture supernatant, increased the activity of MSDH, ATPase (Na⁺-K⁺ATPase, Ca²⁺ATPase) and mitochondrion membrane potential. Conclusion Crocetin could alleviate the disturbance of energy metabolism and decrease the percentage of apoptosis of cardiac myocyte treated with noradrenaline.