A new qualitative and quantitative analytical method of chromatographic fingerprints: total quantum statistical moment

A new qualitative and quantitative analytical method of the chromatographic fingerprints: the Total Quantum Statistical Moment (TQSM) has been eluciated and established according to statistical moment principle. The study was carried out with model drugs as the alcohol extracted liquid for Radix et Rhizoma Rhei (AELRR) by HPLC under the chromatographic conditions that the column was C₁₈, 4.6 mm×250 mm, 5 μm; the detection of wavelengths was set at 254 nm; a solution of acetonitrile: 1% acetic acid water (from 0∶100 to 100∶0) was carried with gradient elution as the mobile phase; the ratio of flow was 1 mL·min^-1; the temperature was 40 ℃. The coefficients were dealt with Excel document. It has been established the expressions that consists of four main parameters: ① total quantum zero moment as AUC_T, area under curve; ② total quantum respondent ratio as AUCPW_T, area under curve per weight; ③ total quantum center moment as MCRT_T, mean chromatographic retention time of total quantum, expressed by _T; ④ total quantum variance as VCRT_T, variance of mean chromatographic retention time of total quantum, expressed by ²_T, by which we have obtained. The TQSM’s parameters of the AELRR, such as AUC_T as 3.273×10⁸ μV·s, AUCPW_T as 2.286×10⁶ μV·s·mg^-1, MCRT_T as 33.50 min, VCRT_T as 484.4 min², and total quantum concentration as 143.2 mg·mL^-1. The total quantum statistic moment can be characterized the curve of chromatographic fingerprints with expressive parameters above, also used to quantitative analyses by AUC_T, to qualitative analyses by AUCPW_T, MCRT_T and VCRT_T.