DETERMINATION OF LIGUSTRAZIN AND FERULIC ACID IN CHUANXIONG RHIZOME BY CAPILLARY ELECTROPHORESIS

AIM:To investigate the feasibility of capillary zone electrophoresis (CZE) in the separation and determination of ligustrazin and ferulic acid in Chuanxiong. METHODS: Based on the effects of internal standard (p-nitrobenzoic acid), electrolyte, sampling method and running voltage, etc. on the quantitative assay of ligustrazin and ferulic acid in samples, the optimal conditions by CZE, UV detection were chosen. RESULTS: The experimental conditions are stated as follows: separation column: uncoated fused-silica capillary (39.5 cm×50 μm ID, 34.8 cm to UV detector); electrolyte: 30 mmol.L^-1 borax, pH 9.43; detection wavelength: 295 nm; sampling: 34 kPa.s; running voltage: 17 kV; temperature: 20℃. The linear ranges of determination for ligustrazin and ferulic acid are 7～423 μg.mL^-1 (γ=0.9995, RSD=0.61%～0.89%) and 4～900 μg.mL^-1 (γ=0.9992, RSD=0.22%～1.15%) respectively, and the recoveries of ligustrazin and ferulic acid are 100.9％±1.9％ (RSD=1.86%, n=6) and 99.8％±1.0％ (RSD=1.03%, n=6), respectively. CONCLUSION: CZE is a good method for the simultaneous separation and determination of ligustrazin and ferulic acid in Chuanxiong.