COLD or HOT natural attribute of Zuojinwan and Fanzuojinwan based on temperature tropism of mice

This study is to investigate the authenticity between COLD and HOT natural attribute in the   famous Chinese medicine formulas —— Zuojinwan (Coptis-Evodia 6∶1) and Fanzuojinwan (Coptis-Evodia 1∶6) based on mice temperature tropism, and establish an objective method to estimate the difference of two natural attribute by using a cold/hot plate differentiating technology.  The results indicated that the COLD   nature Zuojinwan could decrease significantly the remaining rate of HOT-symptom rat on warm pad (P < 0.05).  That was not notable to COLD-symptom rat.  The interference result of COLD-HOT temperature tropism to COLD/HOT symptom rat in Fanzuojinwan was the reverse with the COLD nature Zuojinwan.  Meanwhile, biochemical indicators which are relative to energy metabolism such as ATPase enzyme activity and total anti-oxidant capability (T-AOC), had corresponding change in the organism.  In the study, the COLD and  HOT natural tendency in Zuojinwan and Fanzuojinwan which were composed by the same herbs with different proportion could be expressed qualitatively, quantitatively, objectively and directly with applying animal  temperature tropism, and be verified to philosophical idea of treating disease theory with “expelling heat with cold herbs and cryopathy requiring warm prescription”, not “expelling heat with heat herbs and cryopathy requiring cold prescription” in ancient traditional Chinese medicine, which brings a new approach in investigation of the nature theory of traditional Chinese medicine.