STUDY OF SESQUITERPENE ALKALOIDS FROM TRIPTERYGIUM WILFORDII HOOK.F.

AIM　To study the chemical composition of Tripterygium wilfordii Hook.f. METHODS Column chromatography was used to separate the chemical constituents. UV, IR, MS, HRMS, ¹HNMR, ¹³CNMR (COM and OFR), ¹H-¹H COSY, ¹H-¹³C COSY, 2D-NOESY and ¹H-¹³C COLOC were used to determine the structures of the isolated constituents. RESULTS　Two sesquiterpene alkaloids were isolated and their structures were elucidated as euonine and wilfordconine on the basis of spectral evidence. CONCLUSION Wilfordconine, a new sesquiterpene alkaloid,was shown to be immunosuppressive.