FLUORESENT PROPERTIES AND ISOMERIZATION OF BILIRUBIN IX_α IN AQUEOUS ALKALINE SOLUTION

The intense fluorescence and interesting features of bilirubin IX_α in aqueous alkalinesolution were observed.The fluorescent intensitv(F)increased while the solution was exposed tolight and heat.The effects of other factors on F have also been investigated.The decay curve offluorescence shows that the bilirubin sOlution can give more intense and stable fluorescent signal formore than 30 minutes,and this is very significant to establish a new fluorometry for bilirubin. A darkgreen solid substance with fluorescent activity was isolated from the alkaline bilirubin solution,and wasconfinned to be one of the isomers of bilirubin bv means of UV,IR,FABMS,etc.The isomerizationand the mechanism of variations in fluorescent intensitv of bilirubin were discussed.