8-(N,N-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate inhibited the reduction of cerebral blood flow evoked by 5-HT and KCl in rats

AimTo investigate the impact of 8-(N,N-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate(TMB-8) on the change of cerebral blood flow(CBF) induced by 5-HT or KCl in rats.MethodsThe CBF in rats was measured by a Laser-Doppler flowmeter. The cranial window field was superfused with artificial cerebral spinal fluid containing TMB-8, 5-HT or KCl. Results12.5, 25 and 50 μmol·L^-1 TMB-8 showed no significant effect on rest CBF in rats. 12.5, 25 and 50 μmol·L^-1 TMB-8 apparently inhibited the decline of CBF evoked by 1 or 2 μmol·L^-1 5-HT. When persistent reduction of CBF were evoked by 1 μmol·L^-1 5-HT, TMB-8 markedly increased the CBF in a concentration-dependent manner. The reduction of CBF induced by 20 or 40 mmol·L^-1 KCl was also suppressed by 12.5, 25 and 50 μmol·L^-1 TMB-8. While persistent reduction of CBF was evoked by 20 mmol·L^-1 KCl. TMB-8 markedly increased the CBF in a concentration-dependent manner.ConclusionThese results indicate that TMB-8 is effective in preventing and treating the reduction of CBF induced by 5-HT or KCl, and improved the supply of blood in rat brain during ischemia.