Triterpene glycosides from the aerial parts of Pulsatilla chinensis

To study the chemical constituents of the aerial parts of Pulsatilla chinensis (Bge.) Regel, various chromatography methods were used. Seven triterpene glycosides were isolated from the n-BuOH extract. Their structures were identified as bayogenin 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (1), 3-O-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (2), 3-O-α-L-rhamnopyranosyl (1→2)-α-L-arabinopyranosyl oleanolic acid 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (3), 3-O-α-L-rhamnopyranosyl (1→2)-［β-D-glucopyranosyl (1→4)］-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (4), 3-O-α-L-rhamnopyranosyl (1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (5), hederagenin 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (6) and pulsatilla saponin (7). Among them, compound 1 is a new compound. Compounds 2-6 were isolated from this plant for the first time.