STUDY ON CHEMICAL CONSTITUENTS OF RHIZOME OF Anemone raddeana

AIMTo investigate the chemical constituents of the rhizome of Anemone raddeana Regel, so as to find new active compounds. METHODSThe ethanol extracts of the rhizome of Anemone raddeana were obtained by silica column, HPLC. The structures of the compounds were elucidated by means of physico-chemical method and spectral analysis (IR, FAB-MS, ¹HNMR, ¹³CNMR, DEPT, ¹H-¹H COSY, HMQC, HMBC). RESULTSNine compounds were isolated and identified as 27-hydroxyolean-12(13)-en-28-oic acid-3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside (1), eleutheroside K (2), Oleanolic acid-3-O-α-L-rhamnopyranosyl-(1→2)-［β-D-glucopyranosyl-(1→4)］-α-L-arabinopyranoside (3), betulin (4), betulic acid (5), acetyloleamolic acid (6), evonymitol (7), oleamolic acid (8) and diosgenin (9). CONCLUSIONCompound 1 is a new compound, named raddeanoside 12. Compounds 3～7 were isolated from this plant for the first time.