STUDIES ON ANTITUMOR ANTIBIOTIC 1588 Ⅱ.THE PHYSICOCHEMICAL PROPERTIES AND THE CHARACTERISTICS OF THE MAIN COMPONENTS

In this paper, the UV, IR and NMR spectral characteristics of the antitumor antibiotic 1588 are reported. Besides, it was also resolved on a CM-Sephadex C-25 column with a concentration gradient of ammonium formate into 15 components. Furthermore, the physicochemical and biological properties of the two main components, 1588-74-3 and 1588-74-9 (amounting to 23.4% and 11.3%, respectively), were studied.The paper and thin-layer chromatograms of the acid hydrolysate of these two main components were different from those of bleomycin A₂ and A₅. In addition, these two main components of antibiotic 1588 were also found to be different from other members of the phleomycin-bleomycin group in their UV absorption ratios, pH stabilities and the behavior of chromatography. So, antibiotic 1588 might be considered as a new member of this group.