STRUCTURAL ELUCIDATION OF A NEW TETRAHYDROXYSTILBENE OF RADIX POLYGONI MULTIFLORI PREPARATA AND STUDY ON ITS CARDIOVASCULAR ACTIVITY

AIM　To investigate the cardiovascular active constituents of Radix Polygoni multiflori Preparata. METHODS　Compounds were isolated from the water soluble extract with column chromatography of Sephadex, ODS and HPLC. The compounds were identified on the basis of spectral analysis (IR, EI-MS, FAB-MS, ¹HNMR, ¹³CNMR, 2D-NMR) and phytochemical properties. Its inhibitory effect on the proliferation of bovine vascular smooth muscle cells were bioassayedin vitro. RESULTS　One compound was isolated and identified as: 2,3,5,4′-tetrahydroxystilbene-2-O-(6″-O-α-D-glucopyranosyl)-β-D-glucopyranoside. CONCLUSION　Compound I is a new compound with cardiovascular activity.