Synthesis of 2-amino-4, 5-diarylpyrimidines and their protective effects against oxygen-glucose deprivation in PC12 cells

A series of 6 novel 2-amino-4, 5-diarylpyrimidines were synthesized by the reaction of isoflavones with guanidine.  They were identified by the spectra of IR, ¹H NMR, ¹³C NMR and elemental analysis.  The protective effects of 2-amino-4, 5-diarylpyrimidines against oxygen-glucose deprivation leading by sodium hydrosulfite in PC12 cells were studied by MTT method.  It was shown that 2-amino-4, 5-diarylpyrimidines   exhibited neuroprotective effects against oxygen-glucose deprivation injury in PC12 cells, which could increase   the amount of survival PC12 cells and reduce their injured degree in the model.