HPLC METHOD FOR DETERMINATION OF LORCAINIDE HYDROCHLORIDE IN HUMAN SERUM

An analytical method was established for determination of lorcainide hydrochloride concentration in serum using high performance liquid chromatography(HPL.The stainless steel column was 200 mm × 5. 0 mm ID, packed with YWG C₁₈H₃₇,5μm, The mobile phase consisted of methanol-water-0. 625 mol·L^-1 ammonium acetate.(86:13:1v/v),adjusted to the pH 8. 0 with ammonium hydroxide. The flow rate was 1 ml· min^-1, using diltiazem as internal standard.Chromatography was performed with ultraviolet detector at 226 nm. The recoveries of lorcainide hydrochloride at 40,200 and 400μg,L^-1 were 95.85%, 100.63%and 100.09%, respectively.The within-day and day-to-day RSD of the determinations at concentrations of 100,260,400 and 800μg·L^-1 were less than 7%. Linear calibration curve for lorcainide hydrochloride was measured within the range of 20 to 800μg·L^-1 with correlation coefficient of 0.9996. The detection limit was 5μg·L^-1 in serum. The HPLC method described is suitable for clinical monitoring and pharmacokinetic study of lorcainide hydrochloride.